|
KMID : 0370220100540020091
|
|
Yakhak Hoeji
2010 Volume.54 No. 2 p.91 ~ p.96
|
|
|
Inhibitory Effect of Berberine on TNF--induced U937 Monocytic Cell Adhesion to HT29 Human Colon Epithelial Cells is Mediated through NF-B Rather than PPAR
|
|
Park Su-Young
Lee Gwang-Ik
Kim Il-Yeob
Kim Jung-Ae
|
|
|
Abstract
|
|
|
|
Berberine, an isoquinoline alkaloid, has a wide range of pharmacological effects, including anti-inflammation. It has been reported that berberine inhibits experimental colitis through inhibition of IL-8, and that inhibitory effect of berberine on inflammatory cytokine expression is mediated through peroxisome proliferator activated receptor (PPAR)-. In this study, we examined the effects and action mechanism of berberine on the tumor necrosis factor (TNF)--induced monocyte adhesion to HT29 human colonic epithelial cells, which is commonly used as an in vitro model of inflammatory bowel disease (IBD). Berberine significantly inhibited the TNF--induced monocyte adhesion to HT29, which is similar to the effect of PDTC, a nuclear factor (NF)-B inhibitor. However, ciglitazone and GW, the ligands of PPAR-, did not suppress the TNF--induced monocyte adhesion to HT29 cells. In addition, TNF--induced chemokine expression and NF-B transcriptional activity were significantly inhibited by berberine in a concentration-dependent manner. The results suggest that inhibitory effect of berberine on colitis is mediated through suppression of NF-B and NF-B-dependent chemokine expression.
|
|
|
KEYWORD
|
|
|
berberine, colitis, nuclear factor (NF)-B, peroxisome proliferator activated receptor (PPAR)-, chemokine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|